E2-608

Material Data Sheet

UbcH2/UBE2H Dominant Negative

Price: $180.00
Catalog #: E2-608

UbcH2/UBE2H Dominant Negative

Price: 
180
Quantity: 
100 µg
Data Sheet: 
application/pdf icone2-608-ubch2ube2h-dominant-negative.pdf

This E2 enzyme encodes for the human homolog of the yeast Ubc8 gene. UbcH2 can conjugate ubiquitin to histone H2A in an E3-independent manner in vitro. This E2 enzyme is also involved in the ubiquitination of N-end rule pathway substrates, and can function in conjugation with the E3α ligase enzyme. Additionally, UbcH2 may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Actual concentration will vary with specific lot number.
MW: 
17 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Baboshina O.V., et al. (2001) J. Biol. Chem. 276:39428-39437

Cao PR. et al. (2005) Int. J. Biochem. Cell. Biol. 37 :2088-2097

Dohmen J.R., et al. (1991) Proc. Natl. Acad. Sci. 88:7351-7355

Hasselgren P.O. (1999) Mol. Biol. Rep. 26:71-76

Hobler S.C., et al. (1999) Am. J. Physiol. 276:468-473

Kaiser P., et al. (1995) FEBS. Lett. 377:193-196

Lazarus D.D. et al. (1999) Amer. Physiol. Soc. 277: E332-341

Solomon V., et al. (1998) J. Biol. Chem. 273:25216-25222

Wing S.S., et al. (1992) J. Biol. Chem. 267:6495-6501