E2-603

Material Data Sheet

UbcH1/UBE2K/E2-25K

Price: $229.00
Catalog #: E2-603

UbcH1/UBE2K/E2-25K

Price: 
$229
Quantity: 
100 µg
Data Sheet: 
Biochemically, UBE2K (E2-25K) can catalyze the formation of K48-linked Ubiquitin chains in the absence of an E3 ligase. This enzyme has a unique C-terminal UBA domain that plays a critical role in E1 selectivity and chain synthesis. Recently, UBE2K (or E2-25K or HIP2) has been shown to bind to the N terminus of huntington and may play a role in Huntington disease.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP
MW: 
25 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.