E2-603

Material Data Sheet

UbcH1/UBE2K/E2-25K

Price: $120.00
Catalog #: E2-603

UbcH1/UBE2K/E2-25K

Price: 
$120 $200
Quantity: 
50 µg 100 µg
Data Sheet: 
application/pdf icone2-603-ubch1ube2ke2-25k.pdf
Biochemically, UBE2K (E2-25K) can catalyze the formation of K48-linked ubiquitin chains in the absence of an E3 ligase. This enzyme has a unique C-terminal UBA domain that plays a critical role in E1 selectivity and chain synthesis. Recently, UBE2K (or E2-25K or HIP2) has been shown to bind to the N terminus of huntington and may play a role in Huntington disease.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol. Actual concentration will vary with specific lot number.
MW: 
25 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support conjugation in vitro is 100 nM-1 μM depending on conditions.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Chen Z.J., et al. (1991) J. Biol. Chem. 266:15698-15704

Haldeman M.T., et al. (1997) Biochem. 36:10526-10537

Kalchman M.A.., et al. (1996) J. Biol. Chem. 271:19385-19394

Mastrandrea L.D., et al. (1998) Biochem. 37:9784-9792

Pickart C.M., et al. (1992) J. Biol. Chem. 267:14418-14423

Piotrowski J., et al. (1997) J. Biol. Chem. 272:23712-23721

Song S., et al. (2003) Mol. Cell 12:553-563

Yao T. and Cohen R.E. (2000) J. Biol. Chem. 275:36862-36868