E-800

Material Data Sheet

His6-NEDP1/SENP8

Price: $109.00
Catalog #: E-800

His6-NEDP1/SENP8

Price: 
$109
Quantity: 
50 µg

Also known as: NEDD8-specific protease, SENP8

The covalent modification of proteins by NEDD8 is reversible and is mediated by NEDP1. NEDP1 cleaves residues from the C-terminus of NEDD8 precursor proteins to generate the mature and active form which contains the conserved C-terminal di-glycine. This enzyme also reverse the process of NEDDylation by removing poly-NEDD8 conjugates from substrate proteins. NEDP1 has been shown to deconjugate NEDD8 from CUL2 in vitro and from CUL4A in vivo, and does not show activity against ubiquitin or SUMO proteins. Accession # NP_660205

Product Information

Stock: 
X mg/ml (X mM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT. Concentration will vary with specific Lot #.
MW: 
26.5 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support hydrolysis of substrates in vitro is 50- 500 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Gong L. and Yeh. E.T.H. (1999) J.Biol.Chem. 274:212036-12042

Hemelaar J., et al. (2004) Mol.Cell.Biol. 24: 84-95

Hochstrasser M. (2002) Science. 298: 549-552

Mendoza H.M., et al. (2003) J.Biol.Chem. 278:25637-25643

Reverter D., et al. (2005) 345:141-151

Shen L.N., et al. (2005) EMBO. J. 24:1341-1351

Wada H., et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105

Wu K., et al. (2003) J.Biol.Chem. 278:288882-288891