Material Data Sheet
Also known as: NEDD8-specific protease, SENP8
The covalent modification of proteins by NEDD8 is reversible and is mediated by NEDP1. NEDP1 cleaves residues from the C-terminus of NEDD8 precursor proteins to generate the mature and active form which contains the conserved C-terminal di-glycine. This enzyme also reverse the process of NEDDylation by removing poly-NEDD8 conjugates from substrate proteins. NEDP1 has been shown to deconjugate NEDD8 from CUL2 in vitro and from CUL4A in vivo, and does not show activity against ubiquitin or SUMO proteins. Accession # NP_660205
Stock:X mg/ml (X mM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT. Concentration will vary with specific Lot #.
Purity:> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support hydrolysis of substrates in vitro is 50- 500 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Store at -80°C. Avoid multiple freeze/thaw cycles.
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