E-720

New
Material Data Sheet

3CL Protease, SARS-CoV-2

Price: $351.00
Catalog #: E-720

3CL Protease, SARS-CoV-2

Price: 
$351
Quantity: 
50 µg
Data Sheet: 

The 3CL protease (aka 3CLpro, Mpro or "Main Protease) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide.  3CLpro activity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid.  Along with the CoV-2 Papain-Like Protease (catalog number E-611), 3CLpro presents an attractive target for therapeutic intervention for COVID-19.  Because no human proteases with a similar cleavage specificity are known, inhibitors of 3CLpro are unlikely to cause mechanism-based toxicity.  This recombinant protein is untagged.

Product Information

Stock: 
1.7 mg/ml (50 µM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM EDTA.  
MW: 
34 kDa
Purity: 
>95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue Stain

Use & Storage

Use: 

Recombinant SARS-CoV-2 virus 3CL Protease is a cysteine protease that cleaves viral proteins with sequences including a LQ↓[S/A/G] motif.  Reaction conditions will need to be optimized for each specific application.

Storage: 

Store at -80°C