E-710

Material Data Sheet

His6-SENP2 Catalytic Domain

Price: $109.00
Catalog #: E-710

His6-SENP2 Catalytic Domain

Price: 
$109
Quantity: 
50 µg

Also know as:  SUMO- specific protease 2 catalytic domain

The covalent modification of proteins by SUMO is reversible and is mediated by SENP enzymes. SENPs cleave residues from the C-terminus of SUMO precursor proteins to generate the mature and active form which contains the conserved C-terminal di-glycine. These enzymes also reverse the process of SUMOylation by removing poly-SUMO conjugates from substrate proteins. SENP2 is active against SUMO-1, SUMO-2 and SUMO-3 in vitro but not ubiquitin or NEDD8. N-terminally tagged SENP2 contains the catalytic domain consisting of residues 368-593. Accession # NP_067640.

Product Information

Stock: 
X mg/ml (X mM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT. Concentration will vary with specific Lot #.
MW: 
28.7 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support hydrolysis of substrates in vitro is 50- 500 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Bailey D and O’Hare P. (2004) J.Biol.Chem. 279: 692-703

Chen J., et al. (2004) Mol.Cell.Biol. 24:6021-6028

Chen J., et al. (2005) J.Biol.Chem. 280: 14492-14498

Gong L. and Yeh. E.T.H. (1999) J.Biol.Chem. 274:212036-12042

Kim Y.H., et al. (2005) FEBS Lett. 579: 6272-6278

Xu K., et al. (2005) Biochem. J. 386: 325-330

Yamaguchi T., et al. (2005) Mol.Cell.Biol. 12:5171-5182