Material Data Sheet

His6-SENP1 Catalytic Domain

Price: $125.00
Catalog #: E-700

His6-SENP1 Catalytic Domain

50 μg

Also known as: SUMO-specific Protease 1 catalytic domain

The covalent modification of proteins by SUMO is reversible and is mediated by SENP enzymes. SENPs cleave residues from the C-terminus of SUMO precursor proteins to generate the mature and active form which contains the conserved C-terminal di-glycine. These enzymes also reverse the process of SUMOylation by removing poly-SUMO conjugates from substrate proteins. SENP1 is active against SUMO-1, SUMO-2 and SUMO-3 in vitro but not ubiquitin or NEDD8. N-terminally tagged SENP1 contains the catalytic domain consisting of residues 415-643. Accession # NP_055369.

Product Information

X mg/ml (X mM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT. Concentration will vary with specific Lot #.
29.8 kDa
> 95% by SDS-PAGE

Use & Storage


Typical enzyme concentration to support hydrolysis of substrates in vitro is 50-
500 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is
recommended to achieve maximum activity.

Store at -80°C. Avoid multiple freeze/thaw cycles.



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