E-700

Material Data Sheet

His₆-SENP1 Catalytic Domain

Price: $100.00

Catalog #: E-700

His₆-SENP1 Catalytic Domain

Price:

100

Quantity:

50 µg

Data Sheet:

e-700-his6-senp1-catalytic-domain.pdf

Also known as: SUMO specific protease 1 catalytic domain

The covalent modification of proteins by SUMO is reversible and is mediated by SENP enzymes. SENPs cleave residues from the C-terminus of SUMO precursor proteins to generate the mature and active form which contains the conserved C-terminal di-glycine. These enzymes also reverse the process of SUMOylation by removing poly-SUMO conjugates from substrate proteins. SENP1 is active against SUMO-1, SUMO-2 and SUMO-3 in vitro but not ubiquitin or NEDD8. N-terminally tagged SENP1 contains the catalytic domain consisting of residues 415-643. Accession # NP_055369.

Product Information

Stock:

X mg/ml (X mM) in 50 mM Hepes pH 8.0, 100 mM NaCl, 10% glycerol, 5 mM DTT. Concentration will vary with specific Lot #.

MW:

29.8 kDa

Purity:

> 95% by SDS-PAGE

Use & Storage

Use:

Typical enzyme concentration to support hydrolysis of substrates in vitro is 50- 500 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.