E-621

New
Material Data Sheet

MINDY-3/FAM188A

Price: $285.00
Catalog #: E-621

MINDY-3/FAM188A

Price: 
285
Quantity: 
25 µg
Data Sheet: 

MINDY-3/FAM188A is a member of a newly characterized cysteine protease family of deubiquitinases ("DUBs").  In humans, the MINDY (MIU containing novel DUB family) family has at least four members, with an additional three being MINDY-1/FAM63A, MINDY-2/FAM63B and MINDY-4/FAM188B.  The catalytic domain of MINDY DUBs adopts a conformation that is distinct from other known DUB classes, and these enzymes are highly selective at cleaving K48-linked polyubiquitin chains.  With a predicted molecular weight of 50 kDa, human MINDY-3 is 95% identical to both its murine and rat orthologs.  This recombinant protein is untagged.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
MW: 
50 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Recombinant Human MINDY-3 is a Ubiquitin­specific deconjugating enzyme that cleaves K48-linked polyubiquitin chains.  MINDY-3 will not hydrolyze Ubiquitin-Rhodamine.

Storage: 

Store at -80°C and avoid freeze/thaw cycles