E-620

New
Material Data Sheet

MINDY-2/FAM63B

Price: $285.00
Catalog #: E-620

MINDY-2/FAM63B

Price: 
285
Quantity: 
50 µg
Data Sheet: 

MINDY-2/FAM63B is a member of a newly characterized cysteine protease family of deubiquitinases ("DUBs").  In humans, the MINDY (MIU containing novel DUB family) family has four members, with the additional three being MINDY-1/FAM63A, MINDY-3/FAM188A and MINDY-4/FAM188B.  The catalytic domain of MINDY DUBs adopts a conformation that is distinct from other known DUB classes, and these enzymes are highly selective at cleaving K48-linked polyubiquitin chains.  With a predicted molecular weight of 67 kDa, human MINDY-2 is 82% identical to both its murine and rat orthologs.  This recombinant protein is untagged.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
MW: 
67 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Recombinant Human MINDY-2 is a Ubiquitin-­specific deconjugating enzyme that cleaves K48-linked polyubiquitin chains.  MINDY-2 will not hydrolyze Ubiquitin-AMC or Ubiquitin-Rhodamine.

Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles.