E-619

Material Data Sheet

Josephin-2, His6 tag

Price: $275.00
Catalog #: E-619

Josephin-2, His6 tag

Price: 
275
Quantity: 
50 µg
Data Sheet: 

Josephin-2 is a member of the Machado-Joseph Disease (MJD) family of cysteine proteases, whose other members include Ataxin-3, Ataxin-3-like, and Josephin-1.  188 amino acids long with a predicted molecular weight of 21 kDa, Josephin-2 shares 95% identity with its mouse ortholog.  Josephin-2 is capable of cleaving K11- and to a lesser extent, K63-linked polyubiquitin chains in vitro.  This recombinant enzyme contains a c-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X mM) in 50 µM HEPES pH 7.5, 300 mM NaCl, 10% (v/v) Glycerol,   1 mM DTT
MW: 
22 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain
Storage: 

Store at -80°C and avoid multiple freeze/thaw cycles.