E-618

Material Data Sheet

Josephin-1, His6 tag

Price: $275.00
Catalog #: E-618

Josephin-1, His6 tag

Price: 
275
Quantity: 
25 µg
Data Sheet: 

Josephin-1 is a member of the Machado-Joseph Disease (MJD) family of cysteine proteases, whose other members include Ataxin-3, Ataxin-3-like, and Josephin-2.  202 amino acids long with a predicted molecular weight of 23 kDa, Josephin-1 shares 97% identity with its mouse and rat orthologues.  When ubiquitinated, Josephin-1 is capable of cleaving K48- and K63-linked polyubiquitin chains in vitro.  Biologically, this enzyme may act to increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis.  This recombinant enzyme contains a c-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.0, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
MW: 
24 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain
Storage: 

Store at -80°C and avoid multiple freeze/thaw cycles