Material Data Sheet

USP16, Histag

Price: $225.00
Catalog #: E-612

USP16, Histag

25 µg
Data Sheet: 

USP16 is a deubiquitinating enzyme (DUB) of the C19 peptidase family, USP16 subfamily. Human USP16 has a predicted molecular weight of 94 kDa and is 83% identical to both mouse and rat orthologues. USP16 plays significant roles in cell-cycle progression and Hox gene expression, and USP16 knockdown in HeLa cells results in defects in the mitotic phase of the cell cycle. Studies reveal that histone H2A deubiquitination by USP16 is a prerequisite for phosphorylation of Serine 10 of histone H3 and chromosome segregation during mitosis. USP16 specifically deubiquitinates histone H2A, but not H2B, both in vitro and in vivo. PLK1 serine/threonine kinase is another reported target of USP16. Finally, a chromosomal inversion involving USP16 and RUNX1/AML1 is a causative event in the development of chronic myelomonocytic leukemia (CMML). This recombinant protein contains an N-terminal 6-His tag.

Product Information

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
94 kDa
> 85% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain