Material Data Sheet

Papain-Like Protease, SARS-CoV-2 

Price: $351.00
Catalog #: E-611

Papain-Like Protease, SARS-CoV-2 

50 µg
Data Sheet: 

The Papain-like protease (PLPro) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2 or Wuhan 2019 coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  In other coronaviruses, PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminal end of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISG15ylating activity.  In vivo, this protease antagonizes innate immunity by acting on IFN β and NF- κB signaling pathways.  When used in vitro with polyubiquitin substrates, the enzyme demonstrates a strong preference for K48 linkages.  This protein contains an N-terminal GST tag.

Product Information

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP 
62 kDa
>90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue Stain

Recombinant SARS-CoV-2 virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.   We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 (U-550, U-555) substrates.  Using polyubiquitin chains as a substrate, PLPro demonstrates a preference for K48 linkages.


Store at -80°C