Ubiquitin-like carboxyl-terminal hydrolase 18 (USP18) is a specialized cysteine protease with a predicted molecular weight of 42 kDa. USP18 from Mus musculus is a member of the peptidase C19 family and shares 70% amino acid sequence identity with its human ortholog. Biologically, USP18 plays an important role in antiviral responses and interferon-mediated signaling pathways. Mice lacking the gene are hypersensitive to interferon, have shortened lifespans and pathologic abnormalities of the brain. While it was initially speculated that the cause of the defects resulted from the accumulation of ISG15-conjugated intracellular proteins (a target of USP18 catalytic function), it was later demonstrated that USP18 has multiple signaling roles, at least one of which is independent of its ISGylase activity.This recombinant protein contains residues Ser47-Ser368 of Accession Number Q9WTV6.
Use & Storage
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant murine USP18 concentration of 100-250 nM when using ISG15-AMC (UL-553) as a substrate.
Store at -80°C. Avoid multiple freeze/thaw cycles.