E-594

Material Data Sheet

His6-USP15

Price: $269.00
Catalog #: E-594

His6-USP15

Price: 
$269
Quantity: 
50 µg
Data Sheet: 
application/pdf icone-594.pdf

Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a specialized cysteine protease with a predicted molecular weight of 112 kDa.  USP15 is a member of the peptidase C19 family and the human protein shares 98% amino acid sequence identity with its mouse ortholog.  USP15 is a regulator of the TGFβ signaling pathway, where it may promote signaling strength by deubiquitination of SMAD proteins and/or the TGFβR1.  Other reported targets of USP15 include histone H2B, NFκBIα, MDM2 and human papillomavirus type 16 E6 protein. 

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
MW: 
113 kDa
Purity: 
> 85% by SDS-PAGE

Use & Storage

Use: 

Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP15 concentration of  5-20 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles.