Valosin-containing protein p97/p47 complex-interacting protein, or VCIP135, is a member of the OTU superfamily of Deubiquitinating Enzymes. It is 1222 amino acids (aa) in length with a predicted molecular weight of 134 kDa. Human VCIP135 shares 95% aa sequence identity with the mouse ortholog. In the cell, VCIP135 function requires membrane association and interaction with p97, both of which are inhibited by VCIP135 phosphorylation during mitosis. VCIP135 activity is necessary for VCP-mediated reassembly of Golgi stacks after mitosis. In vitro, VCIP135 efficiently hydrolyzes K11- and K48-linked poly-Ubiquitin chains.
Use & Storage
Reaction conditions will need to be optimized for each specific application. We recommend an initial VCIP135 concentration of 100-500 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Store at -80ºC. Avoid multiple freeze/thaw cycles.