Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a specialized cysteine protease with a predicted molecular weight of 97 kDa. USP13 is a member of the peptidase C19 family and the human protein shares 96% amino acid sequence identity with its mouse ortholog. Recombinant USP13 has weak deubiquitinase activity against unanchored polyubiquitin chains, but will disassemble tetraubiquitin chains in extended reactions, particularly K63 and K48 linkages. Suggested biological targets of USP13 include PTEN, BECN1, MITF, SKP2 and USP10, and roles for USP13 in melanoma and colorectal cancer have been reported. This recombinant protein contains a C-terminal 6-His tag.
Use & Storage
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP13 concentration of 100-500 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Store at -80°C. Avoid multiple freeze/thaw cycles.