E-586

New
Material Data Sheet

His6-USP46/UAF1 Complex

Price: $229.00
Catalog #: E-586

His6-USP46/UAF1 Complex

Price: 
$229
Quantity: 
70 µg
Data Sheet: 

Ubiquitin carboxyl-terminal hydrolase 46 (USP46) is a specialized cysteine protease with a predicted molecular weight of 42 kDa. USP46 is a member of the peptidase C19 family and USP12/USP46 subfamily, and the human protein shares 100% amino acid sequence identity with its mouse ortholog. Like USP1 and USP12, USP46 lacks appreciable activity in the absence of its co-activator UAF1. Addition of UAF1 increases the in vitro activity of USP46 10-fold or more. Biologically, USP46 has been implicated in the regulation of Alpha-Amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid receptors (AMPARs). USP46 is able to deubiquitinate AMPARs both in vivo and in vitro, and by regulating the status of AMPAR ubiquitination and turnover, this deubiquitinase may play an important role in synaptic plasticity and brain function. Recombinant USP46 contains a C-terminal 6-His tag. Recombinant UAF1 also contains a C-terminal 6-His tag. The assembled complex contains 25 μg USP46 and 45 μg UAF1, resulting in a 1:1 stoichiometry.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.8, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
MW: 
43 kDa, 77 kDa
Purity: 
> 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Reaction conditions will need to be optimized for each specific application.  We recommend an initial recombinant human USP46/UAF1 Complex concentration of 20-200 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550U-555) as a substrate.  In vitro, the USP46/UAF1 complex will not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.