E-576

Material Data Sheet

His6-USP19

Price: $265.00
Catalog #: E-576

His6-USP19

Price: 
$265
Quantity: 
50 µg
Data Sheet: 

Ubiquitin specific processing protease 19 (USP19) is a 146 kDa member of the C19 peptidase family and contains two alpha crystallin domains and one MYND-type zinc finger. USP19 plays a role in the rescue of ER-associated protein degradation (ERAD) substrates such as cystic fibrosis transmembrane conductance regulator (CFTR) ΔF508 and T-cell receptor-alpha (TCRα) from proteasomal degradation. USP19 also interacts with the ubiquitin ligases cellular IAP 1 (cIAP1) and cIAP2. Knockdown of USP19 decreases levels of both cIAPs, whereas overexpression of USP19 results in a marked increase in cIAP levels. Although it effectively removes ubiquitin from cIAPs in vitro, USP19 may stabilize cIAP proteins in vivo mainly through deubiquitinase-independent mechanisms. Intriguingly, partial rescue of TCRα (but not CFTR ΔF508) in the ERAD pathway is also observed using a catalytically dead USP19, suggesting that USP19 exerts a non-catalytic function for select substrates in multiple biological pathways. This recombinant protein contains amino acids 1-1290 (UniProt # O94966) and a C-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
MW: 
143 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Reaction conditions will need to be optimized for each specific application. We  recommend an initial USP19 concentration of 10-­100 nM.

Storage: 

Store at -80ºC.  Avoid multiple freeze/thaw cycles.