E-574

Material Data Sheet

OTUD3, Catalytic Domain

Price: $329.00
Catalog #: E-574
OTUD3, Catalytic Domain
Price: 
$329
Quantity: 
50 μg
Data Sheet: 
OTU domain-containing protein 3 (OTUD3) is a specialized cysteine protease and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 45 kDa. The human protein shares 83% amino acid sequence identity with its mouse ortholog. OTUD3 is exceedingly well characterized in terms of the structure-function relationship that results in the enzymes preferred polyubiquitin substrates. This untagged recombinant protein contains the catalytic (OTU) and UBA-like (Ubiquitin Associated) domains of OTUD3, and demonstrates a strong preference for K6- and K11-linked polyubiquitin chains.

Product Information

Stock: 
50 mM Hepes pH 7.5, 100 mM NaCl, 10 % glycerol, and 1 mM TCEP
MW: 
26 kDa
Purity: 
≥ 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue Stain.

Use & Storage

Use: 
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human OTUD3 Catalytic Domain concentration of 0.1-1.0 µM.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Mevissen, T.E.T. et al. (2013) Cell 154: 169–184