E-570

Material Data Sheet

His6-USP28

Price: $269.00
Catalog #: E-570

His6-USP28

Price: 
$269
Quantity: 
100 µg
Data Sheet: 

USP28 is a deubiquitinating enzyme of the C19 peptidase family that regulates MYC stability and DNA damage response checkpoints.  USP28 regulates DNA repair by deubiquitinating and thereby stabilizing CLSPN—a protein that is ubiquitinated by the APC/C E3 ubiquitin ligase in a cell-cycle dependent manner.  USP28 interacts with isoform 1 of FBXW7 in the nucleoplasm, and regulates MYC degradation by counteracting its ubiquitination.  Recently, a potential role in HIF-1α-dependent processes such as angiogenesis and metastasis has been described for this deubiquitinase.  USP28 has been demonstrated to cleave K6, K11, K48, and K63-linked polyubiquitin chains.  This recombinant protein contains a C-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X mM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP
MW: 
123 kDa
Purity: 
>90% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for use in vitro ranges from 0.01-0.5 mM depending on conditions and substrate.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Basserman F., et al. (2008) Cell  134: 256-267

Flügel D., et al.  (2012) Blood   119:1292-301

Popov N., et al. (2007) Cell Cycle  6: 2327-2331

Zhang D., et al. (2006) Cell  126: 529-542