Material Data Sheet

USP1/UAF1 complex, His6 tag

Price: $385.00
Catalog #: E-568

USP1/UAF1 complex, His6 tag

50 µg
Data Sheet: 

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway.  Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA.  USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy.  By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity.  In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains.  Recombinant USP1 protein contains a C-terminal 6-His tag, and glycine-to-alanine substitutions at positions 670 and 671 (UniProt O94782 numbering).  Recombinant UAF1 protein also contains a C-terminal 6-His tag.  The assembled complex contains 25 µg each of USP1 and UAF1, resulting in a 1:1.2 stoichiometry.

Product Information

X mg/ml total protein including USP1 (X µM) and UAF1(X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
77 kDa, 89 kDa
>80% by SDS-PAGE

Use & Storage


Typical enzyme concentration for in vitro deconjugation reactions ranges from 0.01-0.5 µM depending on conditions and substrate.


Store at -80°C. Avoid multiple freeze/thaw cycles.