E-566

Material Data Sheet

His6-UAF1

Price: $269.00
Catalog #: E-566

His6-UAF1

Price: 
$269
Quantity: 
50 µg
Data Sheet: 

UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) is a positive regulator of at least three deubiquitinating enzymes of the C19 peptidase family including USP1, USP12, and USP46.  In the absence of UAF1, all three of these deubiquitinases are almost completely inactive, and available biochemical evidence suggests UAF1 activation occurs by increasing catalytic turnover as opposed to altered substrate affinity (at least in the case of UAF1/USP1) of the enzymes.  Most notably, UAF1 strongly activates USP1 deubiquitination of monoubiquitinated FANCD2 and PCNA, thereby playing important roles in DNA damage response and translesion synthesis.  The UAF1/USP12 complex has recently been described as a negative regulator of Notch signaling.  Disruption of UAF1-dependent USP12 activity interrupts Notch trafficking to the lysosomes, leading to an increased amount of receptor at the cell surface and to higher Notch activity. At the biochemical level, UAF1/USP12 deubiquitinates the non-activated form of Notch in cell culture and in vitro. This recombinant protein contains a C-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X mM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
MW: 
77 kDa
Purity: 
>90% by SDS-PAGE

Use & Storage

Use: 

Recombinant UAF1 activates USP1 (E-564) at 1:1 stoichiometry in in vitro deconjugation reactions utilizing Ubiquitin-AMC.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Cohn M.A., et al. (2009) J Biol. Chem.  284: 5343-5351

Huang T.T., et al. (2006) Nat. Cell Biol.   8: 339-347

Joo H-Y., et al. (2011) Biol. Chem.  286: 7190-7201

Moretti J., et al. (2012) J Biol. Chem.  287: 29429-29441

Williams S.A, et al. (2011) Cell  146: 918–930