E-564

Material Data Sheet

His6-USP1

Price: $249.00
Catalog #: E-564

His6-USP1

Price: 
$249
Quantity: 
50 µg
Data Sheet: 

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway.  Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA.  USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy.  By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity.  In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains.  This recombinant protein contains a C-terminal 6-His tag, and glycine-to-alanine substitutions at positions 670 and 671 (UniProt O94782 numbering).

Product Information

Stock: 
X mg/ml (X mM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
MW: 
89 kDa
Purity: 
>80% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for in vitro deconjugation reactions ranges from 0.01-0.5 mM depending on conditions and substrate.  Requires a 1:1 stoichiometric amount of UAF1 (E-566) for activity.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Cohn M.A., et al. (2007) Mol. Cell  28:786-797

Huang T.T., et al. (2006) Nat. Cell Biol.   8: 339-347

Nijman S.M.B., et al. (2005) Mol. Cell   17:331-339

Williams S.A, et al. (2011) Cell  146: 918–930