E-563

Material Data Sheet

OTUD7B/Cezanne, Catalytic Domain

Price: $195.00
Catalog #: E-563
OTUD7B/Cezanne, Catalytic Domain
Price: 
$195
Quantity: 
50 µg
Data Sheet: 
OTU domain-containing protein 7B (OTUD7B), also known as cellular zinc finger anti-NF-kappa B (Cezanne), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 93 kDa. The human protein shares 90% amino acid sequence identity with its mouse ortholog. Cezanne has been reported to cleave K48-, and K63-linked poly-Ubiquitin chains, and also to display a preference for K11-linked chains. It has been shown to play a role in the negative regulation of NF-κB signaling, possibly by deubiquitination of RIPK1 and TRAF3. This untagged recombinant protein contains the catalytic domain (including the OTU domain) of Cezanne.

Product Information

Stock: 
50 mM Hepes pH 7.5, 100 mM NaCl, 10 % glycerol, and 1 mM TCEP
MW: 
36 kDa

Use & Storage

Use: 
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human Cezanne Catalytic Domain concentration of 0.1-1.0 µM.
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.
Purity: 
≥ 90% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue Stain.

Literature

References: 

Bremm, A. et al. (2010) Nat. Struct. Mol. Biol. 17: 939

Enesa, K. et al. (2008) J. Biol. Chem. 283: 7036

Evans, P.C. et al. (2003) J. Biol. Chem. 278: 23180

Hu, H. et al. (2013) Nature 494: 371