E-562

Material Data Sheet

His6-OTUD7B/Cezanne

Price: $269.00
Catalog #: E-562

His6-OTUD7B/Cezanne

Price: 
$269
Quantity: 
50 µg
Data Sheet: 

OTU domain-containing protein 7B (OTUD7B), also known as cellular zinc finger anti-NF-kappaB  (Cezanne), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 93 kDa (1). The human protein shares 90% amino acid sequence identity with its mouse ortholog.  Cezanne has been reported to cleave K48-, and K63-linked poly-Ubiquitin chains, and also to display a preference for K11-linked chains (1-3). It has been shown to play a role in the negative regulation of NF-κB signaling, possibly by deubiquitination of RIPK1 and TRAF3 (2,4). This recombinant protein contains a C-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X mM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP
MW: 
93 kDa
Purity: 
>85% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for use in vitro ranges from 0.1-1 mM depending on conditions and substrate.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 
  1. Evans, P.C. et al. (2003) J. Biol. Chem. 278: 23180.
  2. Enesa, K. et al. (2008) J. Biol. Chem. 283: 7036.
  3. Bremm, A. et al. (2010) Nat. Struct. Mol. Biol. 17: 939.
  4. Hu, H. et al. (2013) Nature 494:371.