E-556

Material Data Sheet

His6-CYLD, isoform 1

Price: $275.00
Catalog #: E-556

His6-CYLD, isoform 1

Price: 
$275
Quantity: 
50 µg

CYLD is a C67 type peptidase that has endo-deubiquitinase activity, particularly toward K63-linked polyubiquitin chains.  CYLD is important in the regulation of pathways leading to NFkB activation, and thus plays a role in cell survival, proliferation and differentiation.  Negative regulator of Wnt, TNFα, and TLR4 signaling by disassembly of K63-polyubiquitin chains utilized in these pathways.  This recombinant protein contains an N-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES 8.0, 200 mM NaCl, 2 mM DTT
MW: 
108 kDa
Purity: 
>90% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support hydrolysis of substrates is 50-500 nM depending on experimental conditions.  A 15 minute preincubation with 10 mM DTT is recommended to achieve maximum activity. 

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Komander, D., et al. (2008) Mol Cell 29: 45-464

Trompouki E., et al. (2003) Nature 424: 793-796