E-552

Material Data Sheet

His6-USP9x

Price: $295.00
Catalog #: E-552

His6-USP9x

Price: 
$295
Quantity: 
25µg
Data Sheet: 

USP9x (homologue of drosophila Protein Fat Facets) is a deubiquitinating enzyme of the C19 peptidase family.  USP9x is an essential component of TGFß signaling, where it functions at least partially through deubiquitination of SMAD4.  USP9x may also generate important signaling events in pancreatic ductal adenocarcinoma and chronic myelogenous leukemia.  This recombinant protein contains an N-terminal 6-His tag.

Product Information

Stock: 
X mg/ml (X µM) in 50mM Hepes 8.0, 150mM NaCl, 5mM DTT, 10% glycerol
MW: 
292 kDa
Purity: 
> 85% by SDS-PAGE
Host: 
human recombinant

Use & Storage

Use: 

Typical enzyme concentration for use in vitro ranges from 0.05 - 0.5 µM depending on conditions and substrate.  For optimal conditions, include 5-10 mM DTT or TCEP in reaction buffer.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Dupong S., et al (2009) Cell 136: 123-135

Perez-Mancera, P.A., et al (2012) Nature 486: 266-270

Sun H., et al (2011) Blood 117: 3151-3162