E-551

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Material Data Sheet

GST-AMSH-LP (AMSH-like Protease)

Price: $269.00
Catalog #: E-551

GST-AMSH-LP (AMSH-like Protease)

Price: 
$269
Quantity: 
50 µg
Data Sheet: 

AMSH-like protease (AMSH-LP, also known as STAM-Binding Protein-Like 1, STAMBPL1) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). 436 amino acids in length, AMSH-LP is 56% identical and 73% similar to AMSH, an enzyme that functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. Like AMSH, AMSH-LP only cleaves K63-linked poly-Ubiquitin chains in vitro; however, unlike AMSH, AMSH-LP does not appear to associate with STAM protein.  This recombinant protein is full-length and contains an N-terminal GST tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
MW: 
79 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human AMSH-LP concentration of 100-500 nM when using K63-linked poly-Ubiquitin substrates. AMSH-LP does not efficiently utilize Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate, though a signal may be obtained when the enzyme is used at a concentration of 500 nM or higher.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.