E-548B

Material Data Sheet

AMSH

Price: $279.00
Catalog #: E-548B

AMSH

Price: 
$279
Quantity: 
50 µg
Data Sheet: 

AMSH (also known as STAM Binding Protein) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). This enzyme functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes.  AMSH cleaves K63-linked but not K48-linked polyubiquitin chains, and the in vitro activity of AMSH is greatly increased in the presence of STAM protein.  This untagged recombinant protein is full-length. 

Product Information

Stock: 
X mg/ml (X mM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% (v/v) Glycerol,  1 mM TCEP
MW: 
48 kDa
Purity: 
>85% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for use in vitro ranges from 100-500 nM depending on conditions and substrate.  Suggested Reaction Buffer for plate-based assays is 50 mM HEPES, pH 8.0, 100 mM NaCl, and 0.1 mg/ml BSA.  AMSH activity is partially or fully inhibited by DTT at concentrations above 1 mM.

Note: Binds STAM protein with Kd = ~7 µM.  In in vitro assays containing 200 nM AMSH, a ~10-fold increase in the rate of hydrolysis of K63-linked diubiquitin FRET substrate was achieved upon the addition of STAM.  Half-maximal increase occurred at 0.4 µM STAM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.