E-544

Material Data Sheet

USP14

Price: $115.00
Catalog #: E-544

USP14

Price: 
$115
Quantity: 
25 µg
Data Sheet: 

USP14 (Ubiquitin Specific Protease 14) is the human homolog of the yeast Ubp6 protein and is member a of the family of thiol proteases involved in the hydrolysis of ubiquitin C-terminal protein derivatives. It has been reported to have a low-affinity association with the proteasome (PA700, 19S cap particle), which increases its basal enzyme activity. The primary physiological role of USP14 may be the release of ubiquitin from proteosome-bound conjugates. In vitro, the enzyme has a low affinity for ubiquitin and is unable to disassemble poly-ubiquitin protein conjugates. Mice homozygous for spontaneous USP14 mutations develop ataxia and this enzyme may play an important role in regulating synaptic activity in mammals. USP14 may also have a role in colorectal cancer metastases, possibly controlling the fate of proteins that regulate tumor invasion. Accession # NP_005142.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50mM NaCl, 5mM DTT, 1mM EDTA. Actual concentration varies with specific Lot #.
MW: 
56 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Activity was measured using 18 μM Enzyme, 4 μM Ub-AMC in 100mM Hepes pH8.5, 10mM DTT and 5% glycerol at 25°C.
Under these conditions RFU/min/μg = 2.0

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Anderson C., et al. (2005) J.Neurochem. 95:724-731

Leggett D.S., et al. (2002) Mol. Cell. 10:495-507

Hu M., et al. (2002) EMBO.J. 24:3747-3756

Park K.C., et al. (1997) Arch.Biochem.Biophys. 347:78-84

Shinji S., et al. (2006) Oncol.Rep. 15:539-543

Wilkinson K.D., et al. (2001) EMBO. J. 20:5187-5196

Wilson S.M., et al. (2002) Nat. Genet. 32:420-425

Yin L., et al. (2000) Biochem. 39:100001-100010