E-520

Material Data Sheet

His6-USP8

Price: $339.00
Catalog #: E-520

His6-USP8

Price: 
$339
Quantity: 
25 µg
Data Sheet: 

Also known as: His6-USP8 (UBPY)

USP8 (Ubiquitin Specific Protease8) is a growth-regulated deubiquitinating enzyme (DUB) with a role in endosomal sorting of receptor tyrosine kinases (RTKs) such as EGFR in vivo USP8 is a complex protein with a coiled-coil region, non-classical SH3 binding motif, rhodanese and catalytic domains whose individual structures have been determined. The N-terminus has a novel dimerization fold but it is not known if the full-length protein exists as a homodimer. The C-terminus contains the DUB catalytic center and a nearby Zn++ ribbon subdomain which might have a role in poly-Ub binding specificity and substrate-induced conformational changes. USP8 has DUB activity on Ub chains in vitro and does not discriminate between K48- and K63-linked isopeptide bonds. USP8 interacts with several proteins including Hrs binding protein Hbp, Mona/Gads, Ras exchange factor CDC25Mm, GRAIL3 and NRDP1 E3 ligases, and 14-3-3ε. The overall function of USP8 is still unclear but its main role is in endocytosis which influences signaling cascades for mitogenesis, cell survival, differentiation and migration. This protein contains an N-terminal His6-tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Concentration varies with Lot #.
MW: 
131 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for use in vitro ranges from 1-5 μM depending on conditions and substrate.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Alwan H.A. and van Leeuwen,J.E. (2007) J.Biol.Chem. 282:1658-1669

Avvakumov G.V., et al. (2006) J.Biol.Chem. 281:38061-38070

Bache K.G., et al. (2003) J.Biol.Chem. 278:12513-12521

Ballif B.A., et al. (2006) J.Proteome.Res 5:2372-2379

Clague M.J. and Urbe S. (2006) Tren.Cell.Biol. 16:551-559

Harkiolaki M., et al. (2003) EMBO J. 22:2571-2582

Gnesutta N., et al. (2001) J.Biol.Chem. 276:39448-39454

Janssen J. W. G., et al. (1998) Oncogene. 16:1767-1772

Kato M., et al. (2000) J.Biol.Chem. 275:37481-37487

Naviglio S., et al. (1998) EMBO J. 17:3241-3250

Niendorf S., et al. (2007) Mol.Cell.Biol. 27:5029-5039

Mizuno E., et al. (2006) Traffic 7:1017-1031

Soares L., et al. (2004) Nat.Immun. 5:45-54

Row P.E., et al. (2006) J.Biol.Chem. 281:12618-12624