E-519

Material Data Sheet

His6-USP7

Price: $269.00
Catalog #: E-519

His6-USP7

Price: 
$269
Quantity: 
25 µg
Data Sheet: 

USP7 (Herpes-Associated Ubiquitin Specific Protease) is a nuclear protein that was initially identified as a novel p53-interacting protein. The enzyme deubiquitinates p53 thus stabilizing the levels of this key tumor suppressor, and inducing p53-dependent cell growth repression and apoptosis. USP7 also indirectly affects p53 stability by deubiquitinating HDMX, a regulator of HDM2. Full-length USP7 (1102 amino acids) contains an N-terminal p53 binding domain and 2 highly conserved USP active site domains. The C-terminal domain interacts with HSV-1 protein ICP0. This enzyme is able to cleave model substrates including linear Ub fusions, poly- ubiquitinated EBNA1 and poly-ubiquitinated p53. This recombinant protein has an N-terminal 6His tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
MW: 
128 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for use in vitro ranges from  10-100 nM depending on conditions and substrate.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Cheon K.W and Baek K.H. (2006) Intl.J.Oncol. 16:566-577

Everett R.D.,  et al. (1997) EMBO.J. 16:566-577

Holowaty M.N.,  et al. (2003) J.Biol.Chem. 278:47753-47761

Holowaty M.N., et al. (2004) Biochem.Soc.Trans. 32:731-732

Hu M.,  et al. (2002) Cell. 111:1041-1054

Li M.,  et al. (2002) Nature. 416:648-653

Meulmeester E.,  et al. (2005) Mol.Cell. 19:143-144

Nijman S.M.B.,  et al. (2005) Cell. 123:773-786

Saridakis V.,  et al. (2005) Mol.Cell. 18:25-36

Sheng Y., et al. (2006) Nature.Struc.Biol. 13:285-291