E-519

Material Data Sheet

USP7, His6 tag

Price: $269.00
Catalog #: E-519

USP7, His6 tag

Price: 
$269
Quantity: 
25 µg
Data Sheet: 

Ubiquitin Specific Peptidase 7 (USP7), also known as Herpes Virus­-associated Ubiquitin Specific Protease (HAUSP), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family.  It has a predicted molecular weight of 130 kDa. Human USP7 is 1102 amino acids (aa) in length and shares 99% aa sequence identity with the mouse and rat orthologs.  USP7 was initially identified as a p53-­interacting protein that deubiquitinates p53, thereby stabilizing the protein and inducing p53­dependent cell growth arrest and apoptosis.  USP7 also targets the p53 regulatory proteins MDM2, MDMX, and Daxx, the epigenetic regulator Histone 2B, and the transcription factor FoxO4.  Additionally, USP7 interacts with the HSV­-1 immediate early protein ICP0, contributing to the stabilization and transactivation capability of ICP0 during HSV­-1 infection.  This recombinant protein has an N-terminal 6His-tag.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
MW: 
128 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Typical enzyme concentration for use in vitro ranges from 10-100 nM depending on conditions and substrate.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.