E-504

Material Data Sheet

USP2 Catalytic Domain

Price: $129.00
Catalog #: E-504

USP2 Catalytic Domain

Price: 
$129
Quantity: 
50 µg
Data Sheet: 

USP2 (Ubiquitin Specific Protease 2) is a cysteine protease over-expressed in prostate cancer, is androgen-regulated and interacts with and prolongs the half-life of fatty acid synthase (FAS). FAS stabilization is associated with the malignancy of a subset of aggressive prostate cancers. Mdm2 is also a substrate for USP2 indicating that the enzyme may regulate p53-dependent pathways. The in vivo and in vitro oncogenic properties of USP2 are also linked to its deubiquitinating activity. It has been hypothesized that USP2 might stabilize short-lived proteins that accumulate and may act as regulators or effectors in the induction of apoptosis. This recombinant protein contains residues 259-605 representing the catalytic domain (CD) of USP2.

Product Information

Stock: 
 X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 0.5 mM EDTA, 1mM DTT 
MW: 
40 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

USP2 Catalytic Domain is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We suggest an initial USP2 Catalytic Domain concentration of 1-5 nM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

 

Baek S.H., et al (1997) J.Biol.Chem. 272:25560-25565

Catanzariti A.M., et al (2003) Canc.Res. 63:682-688

Graner E., et al (2004) Canc.Cell.3:253-261

Kwon-Yul R., et al (2006) Anal.Biochem. 353:153-155

Nijman S.M.B., et al (2005) Cell. 123:773-786

Priolo C., et al (2006) Canc.Res. 66:8625-8632

Renatus M., et al (2006) Structure. 14:1293-1302

Tirat A., et al (2005) Anal.Biochem. 343:244-255

Nijman S.M.B., et al (2005) Cell. 123:773-786

Ventadour S., et al (2007) J.Biol.Chem. 282:5302-5309