E-400

Material Data Sheet

His6-Apg4b/ATG4B

Price: $138.00
Catalog #: E-400

His6-Apg4b/ATG4B

Price: 
$138
Quantity: 
25 μg
Data Sheet: 
application/pdf iconE400_Apg4b.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl. Actual concentration will vary with specific Lot #.

MW: 
46.5 kDa
Purity: 
> 95% by SDS-PAGE

The Apg4b is a cysteine protease and a member of the autophagin protein family. This enzyme cleaves the C-terminal sequences of the precursor forms of Apg8 proteins (including GABARAP, GATE-16 and MAP-LC3), and regulates the lipidation and delipidation processes of these proteins. This specific cleavage reveals the conserved C-terminal glycine present in mature and active forms of the Apg8 proteins that are conjugated to phosphatidylethanolamine (PE) during autophagy.

Use & Storage

Use: 
Typical enzyme concentration to support hydrolysis of substrates in vitro is 10- 100 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Storage: 
Store at –80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Abeliovich H. and Klionsky D.J. (2001) Euk. Cell. 65: 463-479
Ichimura Y., et al. (2000) Nature. 408: 488-492
Kabeya Y., et al. (2004) J. Cell. Sci. 117: 2805-2812
Marino G., et al. (2003) J. Biol. Chem. 278: 3671-3678
Reggiori F. and Klionsky D.J. (2002) Euk. Cell. 1:11-21
Suguwara K., et al. (2005) J. Biol. Chem. 280: 40058-40065
Tanida I., et al. (2004) J. Biol. Chem. 279: 36268-36276
Tanida I., et al. (2004) J. Biol. Chem. 279: 47704-47710