E-400

Material Data Sheet

His₆-Apg4b/ATG4B

Price: $138.00

Catalog #: E-400

His₆-Apg4b/ATG4B

Price:

$138

Quantity:

25 μg

Data Sheet:

E400_Apg4b.pdf

Stock:

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM EDTA, 2 mM DTT. Actual protein concentration will vary with specific Lot #.

MW:

46.5 kDa

Purity:

> 95% by SDS-PAGE

The Apg4b is a cysteine protease and a member of the autophagin protein family. This enzyme cleaves the C-terminal sequences of the precursor forms of Apg8 proteins (including GABARAP, GATE-16 and MAP-LC3), and regulates the lipidation and delipidation processes of these proteins. This specific cleavage reveals the conserved C-terminal glycine present in mature and active forms of the Apg8 proteins that are conjugated to phosphatidylethanolamine (PE) during autophagy.

Use & Storage

Use:

Typical enzyme concentration to support hydrolysis of substrates in vitro is 10- 100 nM depending on conditions. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.