E-382

Material Data Sheet

His6-PA28 Activator beta subunit

Price: $199.00
Catalog #: E-382

His6-PA28 Activator beta subunit

Price: 
$199
Quantity: 
100 µg

PA28 is a ring-shaped 11S multimeric complex that can bind to the two ends of the 20S proteasome and dramatically stimulates its capacity to hydrolyze small peptides (but not ubiquitinated proteins or large substrates). It functions in immuno-proteasome assembly, is required for antigen processing, and enhances the generation of class I peptides. In mammals, PA28 is composed of two homologous subunits; PA28α and PA28β, both of which are interferon-induced. This protein has been purified from E.coli.

Product Information

Stock: 
 X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 1mM DTT
MW: 
30 kDa, monomer
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

For activation of proteasome activity, use PA28 at 5- to 15-fold molar excess to activate latent 20S. Exact concentrations will vary depending on conditions.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.