E-379

New
Material Data Sheet

20S Proteasome, Cynomolgus monkey

Price: $175.00
Catalog #: E-379

20S Proteasome, Cynomolgus monkey

Price: 
$175
Quantity: 
50 µg
Data Sheet: 

The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins. It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28α/β complex, or the PA28γ complex. The 20S Proteasome is composed of 28 subunits arranged into four stacked rings. The outer rings, containing seven subunits each, are composed of closely-related but non-identical alpha subunits. The amino-terminal tails of the alpha subunits form a gate that restricts substrate entry into the catalytic core. The inner rings, also containing seven subunits each, are composed of closely-related but non-identical beta subunits. The amino-terminal tails of six of the beta subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention. The 20S Proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28. This protein has been purified from erythrocytes collected from the crab-eating macaque, Macaca fascicularis (Cynomolgus monkey).

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.6, 100 mM NaCl, 1 mM DTT
MW: 
700 kDa (estimated)
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

The 20S Proteasome is able to degrade substrates in an ATP-independent manner. It can be activated chemically with SDS (0.035%) or by the addition of PA28. Reaction conditions will need to be optimized for each specific application. We recommend an initial 20S Proteasome concentration of 0.5-5 nM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.