E-376

Material Data Sheet

20S Immunoproteasome, Murine

Price: $275.00
Catalog #: E-376

20S Immunoproteasome, Murine

Price: 
$275
Quantity: 
50 µg
Data Sheet: 

The 20S catalytic core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Immunoproteasomes are modified forms of the constitutive 20S that function in MHC Class I antigen processing. During immunoproteasome assembly, the constitutive catalytic subunits (Delta, Z and X) are replaced by the inducible subunits LMP2, LMP7 and MECL, the former two being encoded by MHC. This protein has been purified from mouse spleen.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 7.6, 100mM NaCl, 1mM DTT
MW: 
700 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue Stain

Use & Storage

Use: 

Typical enzyme concentration for assay is 0.1-1 nM. 20S can be activated by SDS (0.035%) or with PA28 Activator (Cat. # E-380 or E-381).

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.