E-376

Material Data Sheet

20S Immunoproteasome

Price: $250.00
Catalog #: E-376

20S Immunoproteasome

Price: 
$250
Quantity: 
50 μg
Data Sheet: 
application/pdf icon376_50ug_mouse i20S_.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 7.6, 100mM NaCl, 1mM DTT. Actual concentration will vary with specific Lot #.

MW: 
700 kDa
Purity: 
> 95% by SDS-PAGE

The 20S catalytic core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Immunoproteasomes are modified forms of the constitutive 20S that function in MHC Class I antigen processing. During immunoproteasome assembly, the constitutive catalytic subunits (Delta, Z and X) are replaced by the inducible subunits LMP2, LMP7 and MECL, the former two being encoded by MHC. This protein has been purified from mouse spleen.

Use & Storage

Use: 
Typical enzyme concentration for assay is 0.1-1 nM. 20S can be activated by SDS (0.035%) or with PA28 Activator (Cat. # E-380).
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Cardozo C. and Kohanski R.A. (1999) J.Biol.Chem. 273:16764-16770

Foss G.S and Prydz H. (1999) J.Biol.Chem. 274:35196-35202

Hwang L.Y., et al. (2001) Immunol.Res. 24:245-272

Griffen T.A., et al. (1998) J.Exp.Med. 187:97-104

Kesmir C., et al. (2003) Immunogen. 7: 437-449

Kloetzel P.M., et al. (2001) Nat.Rev.Mol.Cell.Biol. 2:179-188

Kloetzel P.M. and Ossendorp E. (2004) Curr.Opin.Immun. 16:76-81

Vad den Eynde B.T and Morel S. (2001) Curr.Opin.Immun. 13:147-32