E-371

New
Material Data Sheet

Ramos Cell 20S Immunoproteasome

Price: $205.00
Catalog #: E-371

Ramos Cell 20S Immunoproteasome

Price: 
205
Quantity: 
25 µg
Data Sheet: 

The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi­complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non­identical subunits arranged into four stacked rings.  However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, β1, β2, and β5, in the two interior rings of the 20S Proteasome are replaced by three IFNγ-­inducible catalytic subunits: β1i/LMP2, β2i/MECl­1, and β5i/ LMP7. The 20S Immunoproteasome is commonly associated with the 19S, PA28 α/β, or the PA28γ regulatory complexes.  20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation.  Selective inhibition of 20S Immunoproteasome proteolytic activity using small molecule inhibitors is being examined for therapeutic intervention in cancer and inflammatory diseases.   The protein complex has been purified from Burkitt’s lymphoma cells (Ramos, RA1, CRL-1596) following stimulation with IFN-γ and IL2.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.6, 100 mM NaCl, 1 mM DTT
MW: 
700 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

The 20S Immunotproteasome is able to degrade substrates in an ATP-independent manner.  It can be activated chemically with 0.035% SDS or by the addition of PA28 proteins.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial 20S Immunoproteasome concentration of 0.5-5.0 nM.

Storage: 

Store at -80ºC.  Avoid multiple freeze/thaw cycles.