E-366

Material Data Sheet

19S Proteasome

Price: $339.00
Catalog #: E-366

19S Proteasome

Price: 
$339
Quantity: 
25 µg
Data Sheet: 

Also known as: 19S Proteasome (PA700)

This highly purified 19S (PA700) proteasome preparation (from transformed HEK cells) can be used in vitro for the activation of 20S proteasome, as well as for deubiquitination of target substrates via associated DUBs, such as RPN11.  The 19S subunit is the ATP-dependant regulatory particle (RP) of the 26S proteasome (E-365), compromised also of a 20S core particle (CP)(E-350, E-360, E-370, E-375) that is responsible for the proteolytic degradation of short-lived and abnormal intracellular proteins. The 19S subunit can bind to one or both ends of the 20S core particle (CP), stimulating peptidase activity by modulating conformational changes and/or facilitating the access of substrates to the proteolytic 20S core.

Product Information

Stock: 
X mg/ml (X μM) in 20mM Hepes pH 7.5, 20mM NaCl, 1mM DTT, 20% glycerol. Concentration varies with lot number.
MW: 
700 kDa
Purity: 
> 95% by SDS-PAGE
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Adams G.N. (1998) Biochem. 37:12927-12932.

DeMartino G.N., et al. (1994) J.Biol.Chem. 269: 20878-20884.

DeMartino G.N., et al. (1996) J.Biol.Chem. 271:3112-3118.

DeMartino G.N., et al. (1996) J.Biol.Chem. 274:22123-22126.

Hendil K.B., et al. (1998) Biochem J. 332:749-754.

Lam Y.A., et al. (1997) J.Biol.Chem. 272:28438-28446.

Strickland E., et al. (2000) J.Biol.Chem. 275:5565-5572.