E-365

Material Data Sheet

26S Proteasome

Price: $175.00

Catalog #: E-365

26S Proteasome

Price:

$175 $300

Quantity:

25 μg 50 μg

Data Sheet:

365_50ug_h26S.pdf

Stock:

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100mM NaCl, 1mM DTT, 2.5 mM Mg- ATP, 20% glycerol. Actual concentration will vary with specific Lot #.

MW:

2500 kDa

Purity:

> 95% by SDS-PAGE

This highly purified 26S proteasome preparation (from transformed HEK cells) can be used in vitro for the degradation of peptide substrates and poly-ubiquitinated proteins. The 26S proteasome is the major non- lysosomal protease in eukaryotic cells principally responsible for the degradation of ubiquitinated substrates with broad specificity, and thus plays a critical role in the regulation of various biological processes. This multi-catalytic protease has a highly ordered structure composed of two sub-complexes - a 20S core particle (CP) and a 19S regulatory particle (RP). The 20S (E-350, E-360, E-370, E-375) forms the proteolytic core containing peptidase activities, and the 19S confers ATP-dependency and ubiquitinated substrate specificity. The 19S (PA700) (E-366) subunits can bind to one or both ends of the 20S, stimulating peptidase activity by modulating conformational changes and/or facilitating the access of substrates to the 20S core

Use & Storage

Use:

The enzyme should be used immediately after thawing, and typical enzyme concentrations for assay are 1-10nM depending on experimental conditions. The enzyme complex is inherently labile and dissociation into free 20S and 19S sub-complexes occurs over time.

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.