E-360

Material Data Sheet

20S Proteasome, human

Price: $189.00
Catalog #: E-360

20S Proteasome, human

Price: 
$189
Quantity: 
50 µg
Data Sheet: 

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. This multi-subunit complex selectively degrades intracellular proteins. The 20S catalytic core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. This activity degrades a variety of peptide substrates and poly-ubiquitinated proteins with broad specificity in non-ATP dependent process. The 20S proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28. This protein has been purified from human erythrocytes, which has been screened and is negative for hepatitis B surface antigen, antibodies to hepatitis C virus, HIV type 1 antigens and antibodies to HIV type 1 and 2

Product Information

Stock: 
X mg/ml in 50 mM HEPES pH 7.6, 100 mM NaCl, 1 mM DTT
MW: 
700 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for assay is 0.1-1 nM. 20S can be activated by SDS (0.035%) or with PA28 (Cat. # E-380 or E-381).

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.