E-355

Material Data Sheet

20S Proteasome

Price: $139.00
Catalog #: E-355

20S Proteasome

Price: 
$139
Quantity: 
50 µg
Data Sheet: 

The 20S Proteasome is the catalytic core component of the multi-complex 26S Proteasome that selectively degrades intracellular proteins. It is commonly associated with regulatory complexes, which include the 19S Proteasome, the PA28 alpha/beta complex, or the PA28 gamma complex. The 20S Proteasome is composed of 28 subunits arranged into four stacked rings. The outer rings, containing seven subunits each, are composed of closely-related but non-identical alpha subunits. The amino-terminal tails of the alpha subunits form a gate that restricts substrate entry into the catalytic core. The inner rings, also containing seven subunits each, are composed of closely-related but non-identical beta subunits. The amino-terminal tails of six of the beta subunits, three per ring, have proteolytic activity. Inhibition of 20S Proteasome proteolytic core activity using small molecule inhibitors is a valuable tool for the functional study of a variety of proteins and for therapeutic intervention. The 20S Proteasome can be activated chemically by the addition of detergent, by the proteinaceous activator PA28 Activator alpha (E-380), or by 19S/PA700 (E-366).

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8, 150mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW: 
700 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for assay is 0.1-1 nM. 20S can be activated by SDS (0.035%) or with PA28 (Cat. # E-380).

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Coux O. (1996) Ann.Rev.Biochem. 65:801-847

Elenich L.A., (1999) Immunogenetics 49:835-842.

Ganoth D., et al. (1988) J.Biol.Chem. 263:12412-12419.

Hartman-Peterson R., et al. (2003) Tren.Biochem.Sci. 28:26-31

McCormack T.A., et al. (1998) Biochem. 37:7792-7800

Ganoth D., et al. (1988) J.Biol.Chem. 263:12412-12419

Hartman-Peterson R., et al. (2003) Tren.Biochem.Sci. 28:26-31

Rechsteiner M., et al. (1993) J.Biol.Chem. 268:6065-6068

Voges D., et al. (1999) Ann.Rev.Biochem. 68:1015-1068

Waxman L., et al. (1987) J.Biol.Chem. 262:2451-2457