E-355

Material Data Sheet

20S Proteasome

Price: $125.00
Catalog #: E-355

20S Proteasome

Price: 
$125
Quantity: 
50 μg
Data Sheet: 
application/pdf icon355_50ug_mouse20S_.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8, 150mM NaCl, 1mM DTT. Actual concentration varies with lot number.

MW: 
700 kDa
Purity: 
> 95% by SDS-PAGE

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes - a 20S core and a 19S regulator. This multi-subunit complex selectively degrades intracellular proteins. The 20S catalytic core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. This activity degrades a variety of peptide substrates and poly-ubiquitinated proteins with broad specificity in non-ATP dependent process. The 20S proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 (E-380) or 19S (PA700)(E-366). The mouse proteasome enzyme is purified from mouse erythrocytes.

Use & Storage

Use: 
Typical enzyme concentration for assay is 0.1-1 nM. 20S can be activated by SDS (0.035%) or with PA28 (Cat. # E-380).
Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Coux O. (1996) Ann.Rev.Biochem. 65:801-847

Elenich L.A., (1999) Immunogenetics 49:835-842.

Ganoth D., et al. (1988) J.Biol.Chem. 263:12412-12419.

Hartman-Peterson R., et al. (2003) Tren.Biochem.Sci. 28:26-31

McCormack T.A., et al. (1998) Biochem. 37:7792-7800

Ganoth D., et al. (1988) J.Biol.Chem. 263:12412-12419

Hartman-Peterson R., et al. (2003) Tren.Biochem.Sci. 28:26-31

Rechsteiner M., et al. (1993) J.Biol.Chem. 268:6065-6068

Voges D., et al. (1999) Ann.Rev.Biochem. 68:1015-1068

Waxman L., et al. (1987) J.Biol.Chem. 262:2451-2457