Material Data Sheet
Quantity:25 µg 50 µg
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. This multi-subunit complex selectively degrades intracellular proteins. The 20S catalytic core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. This activity degrades a variety of peptide substrates and poly-ubiquitinated proteins with broad specificity in non-ATP dependent process. The 20S proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28. This product has been purified from rabbit erythrocytes.
Stock:X mg/ml in 50 mM HEPES pH 7.6, 150mM NaCl, 1mM DTT. Actual concentration will vary with specific Lot #.
Purity:> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration for assay is 0.1-1 nM. 20S can be activated by SDS (0.035%) or with PA28 (Cat. # E-380).
Store at -80°C. Avoid multiple freeze/thaw cycles.
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