E-345

Material Data Sheet

His6-BAP1

Price: $119.00
Catalog #: E-345

His6-BAP1

Price: 
$119
Quantity: 
50 µg
Data Sheet: 

BAP1 (BRCA1 Associated Protein 1) interacts with the RING-FINGER domain of the E3 ligase BRCA1 (Breast Cancer1, early onset protein) which functions as a tumor suppressor in the BRCA1 growth control pathway. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs) and recombinant BAP1 has deubiquitinating activity in vitro. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. It has been demonstrated that BAP1 and BRCA1 associate in vivo and have overlapping sub-nuclear localization patterns. BAP1 appears to be a key regulator of the BRCA1 growth control pathway and has been proposed to be a novel candidate tumor suppressor. This recombinant protein is N-terminally tagged. Accession # NP_004647.

Product Information

Stock: 
X mg/ml (X μM) 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with specific Lot #.
MW: 
81 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration for use in vitro ranges from 1-5 μM depending on conditions and substrate.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Behrends U., et al. (2003) Intl. J.Can. 106:244-251
Boulton S. J., et al. (2006) Biochem.Soc.Trans. 34:633-645
Feunteun J. and Lenoir G.M. (1996) Bioch.Biophy.Acta. 1242:177-180
Jensen D.E., et al. (1998) Oncogene. 16:1097-1112
Jensen D.E., et al. (1999) Canc. Letters 143:S13-S17
Mallory D.L., et al. (2002) EMBO.J 21:6755-6762
Nagase T., et al. (1990) DNA Res. 3:321-329
Nijman S.M.B., et al.(2005) Cell. 123:773-786