E-344

Material Data Sheet

His6-A20/TNFAIP3 Catalytic Domain

Price: $100.00
Catalog #: E-344

His6-A20/TNFAIP3 Catalytic Domain

Price: 
$100
Quantity: 
50 μg
Data Sheet: 
application/pdf icon344_A20cd.pdf
Stock: 

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1mM DTT. Actual concentration varies with specific Lot #.

MW: 
46 kDa
Purity: 
> 95% by SDS-PAGE

Also known as: His6-A20CD (TNFAIP3 catalytic domain)

A20 (TNFα-induced protein 3) is a cytoplasmic zinc finger protein that inhibits NFκB activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NFκB-dependent gene expression by interfering with RIP- or TRAF2- mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NFκB signaling. This catalytic domain (CD) has an N-terminal his tag and residues 1-371 of A20. Accession #
NP_006281.

Use & Storage

Use: 

Typical enzyme concentration to support hydrolysis of poly-ubiquitin chains (K48- or K63-linked) in vitro ranges from 1-5 μM depending on conditions and substrate.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Boone D.L., et al. (2004) Nat. Immun. 5:1052-1060
De Valck D., et al. (1997) Biochem.Biophys.Res.Comm. 238:590-594
Dixit V.M., et al. (1990) J.Biol. Chem. 265:2973-2978
Evans P.C., et al. (2004) Biochem. J. 378:727-734
Heyninck K., et al. (2005) Tren. Biochem. Sci. 1:1-4
Lee E.G., et al. (2000) Science. 289:2350-2354
Lin R., et al (2006) J.Biol. Chem. 281:2095-2103
Mauro C., et al. (2006) J.Biol. Chem. 281:18482-18488
Opipari A.W., et al. (1990) J.Biol. Chem. 265:14705-14708
Song H.Y., et al. (1996) Proc. Natl. Acad. Sci. 93:6721-6725
Stortz P., et al. (2005) Biochem. J. 387:47-55
Wertz I.E., et al. (2004) Nature. 430:694-699