E-340

Material Data Sheet

UCH-L1

Price: $125.00
Catalog #: E-340

UCH-L1

Price: 
$125
Quantity: 
25 µg
Data Sheet: 

Ubiquitin Carboxyl-terminal Esterase L1 (UCH-L1), also known as PGP 9.5, is a deubiquitinating enzyme with a predicted molecular weight of 25 kDa. The human protein shares 95% amino acid sequence identity with its mouse and rat orthologs. UCHL1 is expressed abundantly in neurons, accounting for 1-2% of total soluble proteins in the brain.  It localizes primarily to the cytoplasm, but a subpopulation has been shown to be transiently nuclear.    The levels of free Ubiquitin appear to be partially regulated by UCH-L1 through the hydrolysis of small Ubiquitin chains and the stabilization of monomeric Ubiquitin. Mice lacking functional UCHL1 show neuronal dysfunction and neurodegeneration, and mutations in this enzyme have been linked to Parkinson's disease, suggesting that it is important for proper central nervous system function.  UCH-L1 also likely plays a complex role in cancer.  It has been reported to function as an oncogene in lymphoma, colorectal cancer, and nonsmall cell lung carcinoma.  In contrast, it is thought to function as a tumor suppressor protein in nasopharyngeal and breast cancers.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM EDTA, 2 mM DTT
MW: 
25 kDa
Purity: 
> 95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain

Use & Storage

Use: 

Recombinant Human UCHL1 is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial UCHL1 concentration of 10-50 nM.

Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.