Material Data Sheet
Also known as: His6-Ubiquitin C-terminal Hydrolase L1 (UCH-L1)
Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. UCH-L1 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. The expression of UCH-L1 is highly specific to cells of the neuroendocrine system and their tumors. UCH-L1 variants (Ile93Met) have been linked to Parkinson disease and may participate in processes critical for neuronal health.
Stock:X mg/ml (X μM) in 50 mM HEPES pH 8.0, 2 mM DTT. Actual concentration will vary with specific Lot #.
Purity:> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Store at -80°C. Avoid multiple freeze/thaw cycles.
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