E-335

Material Data Sheet

His6-UCH-L1

Price: $125.00
Catalog #: E-335

His6-UCH-L1

Price: 
$125
Quantity: 
25 µg
Data Sheet: 

Also known as: His6-Ubiquitin C-terminal Hydrolase L1 (UCH-L1)

Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters from the C-terminus of ubiquitin. UCH-L1 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. The expression of UCH-L1 is highly specific to cells of the neuroendocrine system and their tumors. UCH-L1 variants (Ile93Met) have been linked to Parkinson disease and may participate in processes critical for neuronal health.

Product Information

Stock: 
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 2 mM DTT. Actual concentration will vary with specific Lot #.
MW: 
25 kDa
Purity: 
> 95% by SDS-PAGE

Use & Storage

Use: 

Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

Storage: 
Store at -80°C. Avoid multiple freeze/thaw cycles.

Literature

References: 

Day I.N., et al. (1990) Biochem J. 268:521-524
Dang L.C. et al. (1998) Biochem. 37:1868-1879
Johnston S.C., et al. (1999) EMBO.J. 18:3877-3887
Kurihara LJ., et al. (2001) Hum. Mol. Genet. 10:1963-1970
Leroy E., et al., (1998) DNA.Res. 5:397-400
Mayer A.N. and Wilkinson K.D. (1989) Biochem. 28:166-172
Pickart C.M., et al (1985) J.Biol.Chem. 260:7903-7910
Saigoh K., et al. (1999) Nat. Genet. 23:47-51