Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases (known as the peptidase C12 family) that catalyze the hydrolysis of amides, esters and thioesters from the c-terminal end of Ubiquitin. Human UCH-L5 (also known as UCH37) is 329 amino acids long with a predicted weight of 38 kDa, and shares 97% sequence identity with its mouse ortholog. UCH-L5 is found associated with the 19S regulatory complex of the 26S proteasome, where it functions to cleave K48-linked poly-Ubiquitin chains from Ubiquitinated substrates. UCH-L5 is also found as a component of the INO80 complex, where its activity is regulated by a mechanism involving ADRM1.
Use & Storage
Reaction conditions will need to be optimized for each specific application. We recommend an initial UCH-L5 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Store at -80°C. Avoid multiple freeze/thaw cycles.