E-327

Material Data Sheet

UCH-L5/UCH37

Price: $129.00
Catalog #: E-327

UCH-L5/UCH37

Price: 
$129
Quantity: 
25 µg
Data Sheet: 

Ubiquitin C-terminal hydrolases are a family of cysteine hydrolases (known as the peptidase C12 family) that catalyze the hydrolysis of amides, esters and thioesters from the c-terminal end of Ubiquitin.  Human UCH-L5 (also known as UCH37) is 329 amino acids long with a predicted weight of 38 kDa, and shares 97% sequence identity with its mouse ortholog.  UCH-L5 is found associated with the 19S regulatory complex of the 26S proteasome, where it functions to cleave K48-linked poly-Ubiquitin chains from Ubiquitinated substrates.  UCH-L5 is also found as a component of the INO80 complex, where its activity is regulated by a mechanism involving ADRM1.

Product Information

Stock: 
X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
MW: 
38 kDa
Purity: 
> 90% by SDS-PAGE

Use & Storage

Use: 

Reaction conditions will need to be optimized for each specific application.  We recommend an initial UCH-L5 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)  

Storage: 

Store at -80°C.  Avoid multiple freeze/thaw cycles.